Cell Lines and Cell Cultures
Genscript Corporation CHO-K1/ICOS Stable Cell Line
The protein encoded by this gene belongs to the CD28 and CTLA-4 cell-surface receptor family ICOS (Inducible T-cell COStimulator) is expressed on activated T cells It forms homodimers and plays an important role in cell-cell signaling immune responses and regulation of cell proliferation It is thought to be important for Th2 cells in particular
Genscript Corporation CHO-K1/cyno PD-1 Stable Cell L
Recombinant CHO-K1 cells stably overexpress cynomolgus programmed cell death protein 1 (PD-1) on the surface The surface expression of PD-1 is validated by FACS analysis
Genscript Corporation CHO-K1/FPR1/G15 Stable Cell L
The formyl peptide receptors (FPR) are a class of G protein-coupled receptors involved in chemotaxis FPR1 is a Gi-coupled GPCR FPR1 is expressed in the vasculature secretory epithelial cells neurons and other tissues FPR is involved in host defense against bacterial infection and in the clearance of damaged cells
Genscript Corporation CHO-K1/NPS1b/G15 Stable Cell
Recombinant CHO-K1 cells stably overexpress human neuropeptide S receptor 1 (NPSR1) isoform B on the surface and contain high levels of G protein G s and G q to couple with the receptor in downstream signaling pathways
Genscript Corporation CHO-K1/D2/G15 Stable Cell Lin
Dopamine is the predominant catecholamine neurotransmitter found in mammalian brain where it controls a variety of functions including locomotor activity cognition emotion positive reinforcement food intake and endocrine regulation It also plays multiple roles in the periphery as a modulator of cardiovascular function catecholamine release hormone secretion vascular tone renal function and gastrointestinal motility The dopamine receptor family consists of five members which are classified into two groups D1-like (D1 and D5) and D2-like (D2 D3 and D4) Dopamine receptor 2 is mainly expressed in the brain It has splicing variants D2L and D2S D2R receptor is implicated in a number of neurological and psychiatric conditions Drugs acting at dopamine D2 receptors (D2R) are commonly used to alleviate symptoms for Parkinson s disease schizophrenia and depression
Genscript Corporation CHO-K1/CD200 R1 Stable Cell Li
Cell surface glycoprotein CD200 receptor 1 is a protein that in humans is encoded by the CD200R1 gene This gene encodes a receptor for the OX-2 membrane glycoprotein Both the receptor and substrate are cell surface glycoproteins containing two immunoglobulin-like domains This receptor is restricted to the surfaces of myeloid lineage cells and the receptor-substrate interaction may function as a myeloid downregulatory signal Mouse studies of a related gene suggest that this interaction may control myeloid function in a tissue-specific manner
Genscript Corporation HEK293/5-HT2C Stable Cell Line
5-Hydroxytryptamine (5-HT also commonly known as serotonin) is synthesized in enterochromaffin cells in the intestine and in serotonergic nerve terminals In the periphery 5-HT mediates gastrointestinal motility platelet aggregation and contraction of blood vessels Many functions of the central nervous system are influenced by 5-HT including sleep motor activity sensory perception arousal and appetite A family of 12 GPCRs and one ion channel mediate the biological effects of 5-HT (Hoyer et al 1994) 5HT2C is expressed in the brain and spinal cord especially the choroid plexus 5HT2C receptor agonists may have important clinical value in the treatment of mental and eating disorders such as depression panic anxiety OCD bulimia and obesity GenScript s cloned human 5-HT2C expressing cell line is generated in the CHO-K1 host
Genscript Corporation CHO-K1/OX2 Stable Cell Line
Recombinant CHO-K1 cells stably overexpress human hypocretin (orexin) receptor 2 (OX2) on the surface and contain high levels of G protein G q to couple with the receptor in downstream signaling pathways
Genscript Corporation CHO-K1/M5 Stable Cell Line
Muscarinic acetylcholine receptors belong to a superfamily of seven-TM-domain receptors that interact with G-proteins to initiate intracellular responses Five muscarinic receptor subtypes have been identified named M1 through M5 Receptors of the M5 receptor subtype couple through the G sub q/11 /sub class of G-proteins and activate the phospholipase C pathway Activation of this pathway in turn leads to increases in free intracellular calcium levels as inositol triphosphate mediates release of calcium from the endoplasmic reticulum RT-PCR reveals that M5 mRNA is quite uniformly expressed in brain However there is little data regarding the expression and function of the M5 receptor in peripheral tissues Currently it is clear that the M5 receptor due to the high likelihood that its distribution is restricted to the CNS probably plays a discrete role in dopaminergic transmission Although the identification of M5 expression in salivary glands and iris-ciliary muscle sugge
Genscript Corporation CHO-K1/CD80 Stable Cell Line
Cluster of Differentiation 80 (also CD80 and B7-1) is a protein found on activated B cells and monocytes that provides a costimulatory signal necessary for T cell activation and survival It is the ligand for two different proteins on the T cell surface CD28 (for autoregulation and intercellular association) and CTLA-4 (for attenuation of regulation and cellular disassociation) CD80 works in tandem with CD86 to prime T cells
Genscript Corporation CHO-K1/TIGIT Stable Cell Line
TIGIT (T cell immunoreceptor with Ig and ITIM domains) was recently characterized as an inhibitory receptor which is expressed mainly on NK Treg CD8 T and CD4 T cells TIGIT harbors an immunoglobulin tail tyrosine (ITT)-like phosphorylation motif and an ITIM (immunoreceptor tyrosine-based inhibition motif) in its cytoplasmic tail The poliovirus receptor (PVR or CD155) was identified as the physical ligand of TIGIT with high affinity and PVRL2 (Nectin2 or CD112) also binds to TIGIT with a weaker binding capacity TIGIT/PVR engagement suppresses T cell activation through IL-10 secretion mediated by dendritic cells Furthermore TIGIT also exerts an intrinsic inhibitory function to T cell activation
Genscript Corporation CHO-K1/cyno 4-1BB Stable Cell
4-1BB is a member of the tumor necrosis factor (TNF) receptor family Its alternative names are tumor necrosis factor receptor superfamily member 9 (TNFRSF9) CD137 and induced by lymphocyte activation (ILA) It is currently of interest to immunologists as a co-stimulatory immune checkpoint molecule 4-1BB can be expressed by activated T cells but to a larger extent on CD8 than on CD4 T cells In addition 4-1BB expression is found on dendritic cells follicular dendritic cells natural killer cells granulocytes and cells of blood vessel walls at sites of inflammation
Genscript Corporation CHO-K1/NK1 Stable Cell Line
Tachykinins are peptides sharing the common C-terminal amino acid sequence Phe-X-Gly-Leu-Met-NH sub 2 /sub This neuropeptide family is composed of substance P neurokinin A and neurokinin B which are widely distributed in mammalian central and peripheral nervous systems It plays a role as both a neurotransmitter and a neuromodulator Their actions are mediated by binding with three distinct receptors namely NK1 NK2 and NK3 NK1 has high affinity with substance P In the CNS NK1 has been implicated to play a role in regulating neuronal survival and degeneration In the cardiovascular system NK1 mediates endothelium-dependent vasodilatation and plasma protein extravasations In the gastrointestinal system NK1 receptors mediate intestinal motility secretion and vascular functions SP-NK1 receptor communication is also involved in glioma development and progression NK1 receptor antagonists may have several therapeutic applications in diseases mediated by tachykinins such as
Genscript Corporation CHO-K1/GALR2 Stable Cell Line
Recombinant CHO-K1 cells stably overexpress human galanin receptor 2 (GALR2) on the surface and contain high levels of G protein G q to couple with the receptor in downstream signaling pathways
Genscript Corporation CHO-K1/PAR4 Stable Cell Line
Protease-activated receptor (PAR)-4 is a member of a unique family of GPCRs The protease-activated receptors (PARs) that are activated by proteolytic cleavage of the N-terminal domain of the receptor reveal a tethered ligand The PAR family consists of 4 receptors PAR1 and PAR3 are activated by thrombin and PAR2 and PAR4 are activated by several serine proteases (Macfarlane et al 2001) PAR4 is a recently identified low-affinity thrombin receptor that plays a pathophysiological role in many types of tissues including the lung Mice lacking PAR4 are protected from mesenteric arteriole thrombosis indicating that PAR4 is a potential target for treatment of thrombosis in humans